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. 2023 Oct 23;24(20):15489. doi: 10.3390/ijms242015489

Figure 3.

Figure 3

IL8 overexpression enhances liver injury and oxidative stress in the livers of HFD-fed mice. (A) Serum ALT and AST levels (n = 5/group). (B) Body weight and liver weight (n = 5/group). (C,D) Paraffin-embedded liver sections were subjected to the TUNEL assay (panel C) and immunohistochemical analyses of MDA and 4-HNE (panel D) (n = 5/group). Representative images of each staining are presented (left). Scale bars indicate 200 μm. Quantification of the area positive for each staining is illustrated in graphs (right). (E) Mouse liver homogenates were subjected to RT-qPCR analysis of the genes involved in NOX2 complex formation and neutrophil oxidative burst. (n = 5/group). (F) Immunoblot analysis of mouse liver homogenates. Values represent the mean ± standard error of the mean (SEM). Statistical evaluation was performed using Student’s t-tests (** p < 0.01). 4-HNE, 4-hydroxynonenal; ALT, alanine transaminase; AST, aspartate transaminase; HFD, high-fat diet; IL8, interleukin 8; MDA, malondialdehyde; NOX2, NADPH oxidase 2; RT-qPCR, reverse transcription-quantitative polymerase chain reaction.