CA9 protein distribution in murine DRG neuronal cell culture under normoxic and hypoxic conditions. (A–D) Representative photomicrographs of CA9+ DRG neurons of old WT ((A,C), full arrowheads) and old GLA KO mice ((B,D), full arrowheads) cultivated for 24 h under normoxic and hypoxic conditions. (E) Quantification of CA9+ DRG neurons of old WT (○, n = 4 under all conditions) and old GLA KO (●, n = 4 under all conditions) mice under normoxic and hypoxic conditions. Abbreviations: CA9: carbonic anhydrase 9, GLA KO: α-galactosidase A knock-out, WT: wild type. Scale bar: 100 µm. Statistics: One-way ANOVA with Tukey’s multiple comparison test.