TABLE 1.
Porcine Mycoplasma and Acholeplasma strains used in this study and their reaction in the nested PCR assay
| Species | Strain | Sourcea | PCR result
|
||
|---|---|---|---|---|---|
| Outer | Inner | Nested | |||
| M. hyopneumoniae | ATCC 25934 | ATCC (type strain) | + | + | + |
| NCTC10110 | NCTC (type strain) | + | + | + | |
| J (JF 184) | UK | + | + | + | |
| BQ 14 | France | + | +b | + | |
| EP-S 924 | Switzerland | + | +b | + | |
| EP-S 938 | Switzerland | + | + | + | |
| EP-S 939 | Switzerland | + | + | + | |
| EP-S 946 | Switzerland | + | + | + | |
| EP-S 223 | Switzerland | + | + | + | |
| 232 | Camden, NSW, Australia | + | + | + | |
| YZ | Unknown | + | +b | + | |
| Beaufort | Camden, NSW, Australia | + | +b | + | |
| Sue | Camden, NSW, Australia | + | + | + | |
| C173512 | Camden, NSW, Australia | + | + | + | |
| OMZ407 | Camden, NSW, Australia | + | + | + | |
| Hungary 1 | Budapest, Hungary | + | +b | + | |
| M. flocculare | Ms 42 | MRC (type strain) | − | − | − |
| ZH1 | Zürich, Switzerland | − | − | − | |
| ZH2 | Zürich, Switzerland | − | − | − | |
| JF1628 | Cambridge, UK | − | − | − | |
| JF7337 | Cambridge, UK | − | − | − | |
| M. hyorhinis | BTS-7 | MRC (type strain) | − | − | − |
| SEP200 | Stockholm, Sweden | − | − | − | |
| F44 | Stockholm, Sweden | − | − | − | |
| 31-53 | Ottawa, Canada | − | − | − | |
| M. hyosynoviae | S16 | MRC (type strain) | − | − | − |
| M60 | Stockholm, Sweden | − | − | − | |
| A. axanthum | S-743 | MRC (type strain) | − | − | − |
| A. granularum | BTS-39 | MRC (type strain) | − | − | − |
| A. laidlawii | PG8 | MRC (type strain) | − | − | − |
a
ATCC, American Type Culture Collection (Rockville, Md.); NCTC, National Collection of Type Cultures (London, Great Britain); UK, United Kingdom; NSW, New South Wales; MRC, Mycoplasma Reference Center (Aarhus, Denmark).
b
The DNA sequence of the 808-bp fragment was determined with the Taq DyeDeoxy Terminator Cycle sequencing kit (Applied Biosystems/Perkin-Elmer Cetus) and oligonucleotides MHP950-2L and MHP950-2R.