TABLE 4.
Results for air samples analyzed with a nested PCR to detect DNA of M. hyopneumoniae
| Sample type and vol (liters) | No. positive/no. tested (%) in:
|
Total | No. of negative control samples positive/no. tested (%) | |
|---|---|---|---|---|
| Reinfected SPF herd without acute problems | Herd with acute respiratory problems | |||
| Air | ||||
| 18.3–20.0 | 0/1 (0) | 4/5 (80.0) | 6 | |
| 183.0–200.0 | 0/13 (0) | 7/16 (43.8) | 29 | 0/5 (0) |
| >1,000.0 | 0/2 (0) | 1/3 (33.3) | 5 | 0/1 (0) |
| Cumulative (1,098–1,200)a | 0/0 (0) | 3/4 (75.0) | 4 | |
| Total (by farm status) | 0/16 (0)c | 15/28 (53.6)d | 44 | |
| Dust | 0/0 (0) | 1/4 (25.0)b | 4 | |
a
The same filter was used at six different locations in one room, sampling 183 to 200 liters at each.
b
The result for one dust sample was not interpretable.
c
Zero percent of farms were positive.
d
Eighty percent of farms were positive.