Figure 5.

Schematic overview of the scFBA workflow. Single-cell RNA-seq and bulk RNA-seq are performed on patient-derived organoids or xenografts. Extracellular metabolite exchange rates are also measured. A template metabolic network is imported from a public database. The bulk RNA and flux data are integrated to form a population model. Single-cell networks can be computed by incorporating bulk constraints based on bulk data, and single-cell constraints based on the single-cell RNA-seq data. Figure taken from [22].