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Purification of the recombinant low-specificity l-TA of Pseudomonas sp. strain NCIMB 10558a
| Step | Total activity (U) | Total protein (mg) | Sp act (U/mg) | Purification (fold) | Recovery (%) |
|---|---|---|---|---|---|
| Cell extract | 10,812 | 2,120 | 5.1 | 1.0 | 100 |
| Butyl-Toyopearl | 5,056 | 477 | 10.6 | 2.1 | 47 |
| DEAE-Toyopearl | 4,346 | 106 | 41.0 | 8.0 | 40 |
Threonine aldolase activity was determined with l-threonine as a substrate according to the method of Paz et al. (20) as described in Materials and Methods.
