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. 2023 Oct 19;9(10):1028. doi: 10.3390/jof9101028

Table 1.

Different extraction methods for DF used on different mushroom varieties.

Extraction
Methods
Materials Extraction
Conditions
Extraction Features Reference
Physical method Pressurized hot water Pleurotus sajor-caju 140 °C, 0.92 MPa, and 40 min Water as a solvent, low cost, but poor impurity removal [46]
Ultrasound-assisted Agaricus bisporus 15 min, 100 mm amplitude,
and 1 h of precipitation in 80% ethanol
Less time-consuming and highly efficient, but high cost and little capacity [47]
Microwave Cordyceps gunnii
mycelia
1:20 (w/v), 70 °C, 280 W, 5 min High extraction efficiency, short time and low energy input, but the microwave power and microwave time should be strictly controlled [48]
Biological method Enzymatic Schizophyllum commune α-amylase, 100 °C, 30 min; protease 60 °C, 30 min High specificity of enzyme is needed, and the extraction conditions must be strictly controlled [49]
Chemical method Alkaline Coprinus comatus 2% NaOH in a ratio of 1:15, 85 °C, 2 h High yield, but may degrade some compounds [50]
Acid Lentinula edodes stipe 100 °C, 2 h; 0.8 M
trichloroacetic acid, 4 °C,
3 h
High yield, but may produce some byproducts [51]
Combined method Hot water and alkaline Cookeina tricholoma 98 °C, 4 h; 2% KOH (w/v 1:4), 98 °C, 4 h High yield and purity, but time-consuming [52]
Acid–alkaline combined Pleurotus eryngii 0.1 M H2SO4 (1:10 w/v), 60 °C, 2 h; 0.25 M NaOH (1:8 w/v), 60 °C, 2 h Higher purity, low cost, but may cause excessive degradation [53]