FIG. 2.
Plasmids pGADegl3, pCLegl3, and pAGegl3. Relevant gene locations are indicated. See the text for details on the construction of plasmids. ADH1p and ADH1t, promoter and terminator of the S. cerevisiae ADH1 gene, respectively; LEU2, LEU2 gene of S. cerevisiae; 2μ ori, origin of replication of the 2μm plasmid; pUC ori, replication origin of the E. coli pUC18 plasmid; hCMVp, promoter of the hCMV gene; SV40p and SV40t, promoter and terminator of the simian virus 40 (SV40) gene, respectively; neor, neomycin resistance gene of Tn5 conferring G418 resistance in Schizosaccharomyces pombe; pBR ori, origin of replication of the E. coli pBR322 plasmid; Ptac, tac promoter; Trrn, rrnB terminator; Ampr, ampicillin resistance gene.