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. 1998 Feb;64(2):555–563. doi: 10.1128/aem.64.2.555-563.1998

FIG. 8.

FIG. 8

SDS-PAGE and immunostaining of extracellular EG IIIs secreted by S. cerevisiae and Schizosaccharomyces pombe and intracellular EG IIIs produced by E. coli. (A) Purified T. reesei EG III (lane 1), culture supernatants of Schizosaccharomyces pombe SP-cmv-egl3 (lane 2), the control Schizosaccharomyces pombe (lane 3), S. cerevisiae SC-adh-egl3 (lane 4), the control S. cerevisiae (lane 5), crude extracts of E. coli 105-AG-egl3 (lane 6), the control E. coli (lane 7), and the soluble fraction (lane 8) and the cell debris fraction (lane 9) of the crude extract of E. coli 105-AG-egl3. (B) Purified T. reesei EG III (lane 1), recombinant EG III from Schizosaccharomyces pombe (lanes 2 to 4), and recombinant EG III from S. cerevisiae (lanes 5 to 7). Lanes 2 and 5 were untreated, lanes 3 and 6 were treated with endo H, and lanes 4 and 7 were treated with endo H and α-mannosidase.