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Effect of mycelial biomass concentration and time course of extracellular mannanase formation in S. rolfsii CBS 191.62. Washed, glycerol-grown mycelia were transferred to basal medium containing 10 mM cellobiose. The initial pH was 4.5; incubation was done at 30°C and 150 rpm. Mycelium concentrations: □, 0.5 mg (dry weight) per ml; ▵, 1 mg/ml; ▪, 2 mg/ml. ×, blank (no carbohydrate added).
