FIG. 4.

Endoglycanases induced in resting mycelia of S. rolfsii CBS 191.62 (2 mg [dry weight] per ml) by α-cellulose (lane 1), bacterial cellulose (lane 2), locust bean galactomannan (lane 3), cellobiose (lane 4), xylobiose (lane 5), and l-sorbose (lane 6) after 24 h of incubation. The concentrations of the inducers were 1.0 mg/ml for polysaccharides and 3.0 mM for soluble sugars. Proteins were resolved by IEF, and enzyme activities were detected by a zymogram technique using RBB-mannan (A), RBB-xylan (B), RBB-carboxymethyl cellulose (C), and methylumbelliferyl-β-d-cellobioside (D) as substrates. pI values are given for the main enzyme components next to the arrows.