TABLE 1.
Compoundb | Activity (U/ml) of:
|
||
---|---|---|---|
Mannanase | Xylanase | Endoglucanase | |
None (blank) | 0.63 | 0.22 | 0.08 |
Monosaccharides | |||
l-Arabinose | 0.63 | 0.18 | 0.08 |
d-Xylose | 0.93 | 0.33 | 0.33 |
d-Glucose | 0.63 | 0.27 | 0.06 |
d-Galactose | 0.64 | 0.32 | 0.01 |
d-Mannose | 0.69 | 0.24 | 0.05 |
l-Sorbose | 3.00 | 0.80 | 1.68 |
Disaccharides | |||
Xylobiose (Xyl-β-1,4-Xyl) | 2.57 | 1.08 | 2.37 |
Methyl-β-d-xyloside | 0.82 | 0.29 | 0.28 |
Sophorose (Glc-β-1,2-Glc) | 3.26 | 0.73 | 2.11 |
Laminaribiose (Glc-β-1,3-Glc) | 2.97 | 0.71 | 1.26 |
Cellobiose (Glc-β-1,4-Glc) | 3.68 | 0.92 | 3.67 |
Gentiobiose (Glc-β-1,6-Glc) | 2.39 | 0.58 | 1.02 |
Galactobiose (Gal-β-1,4-Gal) | 1.16 | 0.51 | 0.17 |
Mannobiose (Man-β-1,4-Man) | 1.58 | 0.53 | 0.89 |
Galactosyl-β-1,4-mannose | 3.08 | 0.95 | 2.45 |
Lactose (Gal-β-1,4-Glc) | 2.80 | 1.14 | 2.89 |
Polysaccharides | |||
Xylan beechwood | 2.90 | 1.24 | 2.91 |
Xylan birchwood | 0.94 | 0.21 | 0.31 |
Carboxymethyl cellulose | 3.69 | 1.45 | 4.03 |
α-Cellulose | 7.57 | 9.70 | 23.82 |
Bacterial cellulose | 9.15 | 11.93 | 29.04 |
Ivory nut mannan | 3.33 | 0.34 | 1.10 |
Konjac glucomannan | 3.73 | 0.98 | 4.22 |
Guar galactomannan | 3.68 | 1.64 | 4.24 |
Locust bean galactomannan | 3.27 | 1.18 | 3.33 |
The mycelium concentration was 2 mg (dry weight) per ml.
Concentrations: sugars, 3.0 mM; polysaccharides, 1.0 mg/ml. In the control (blank), no carbohydrate was added to the basal medium. In addition to the substrates shown here, the following carbohydrates were tested and resulted in enzyme activities corresponding to the values obtained in the control experiment: d-ribose, d-lyxose, d-fructose, d-mannitol, d-sorbitol, d-glucuronic acid, lactulose, lactitol, lactobionic acid, and sucrose.