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. 2023 Oct 4;11(10):2487. doi: 10.3390/microorganisms11102487

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Shiga toxin (Stx) 2a A subunit and/or its A1 fragment after exposure to fractions of stool supernatant of a healthy individual. This representative immunoblot shows the form (intact or cleaved) of Stx2a after its incubation (30 min) with selected fractions (Fr. 3, 4, 6 to 8) of sterile-filtered supernatant derived from human stool (SF-SN HS) which were not able to cleave, and Fr. 5 which clearly did cleave. Pure Stx2a and trypsin-cleaved (T-cl.) Stx2a served as references. Bands showcasing the whole A subunit or the A1 fragment of Stx2a are indicated by arrows. Molecular weight (MW) is indicated in kilodaltons (kDa) based on the migration of the molecular markers.