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. 2023 Oct 17;28(20):7138. doi: 10.3390/molecules28207138

Figure 6.

Figure 6

FRET between FAD bound to the human recombinant Cb5R with a His6-tag and Aβ(1–42) HiLyteTM-Fluor555. (A) Emission spectrum of the human recombinant Cb5R with a His6-tag with an excitation wavelength of 460 nm (black line) and absorption spectrum of Aβ(1–42) HiLyteTM-Fluor555 (Aβ*555, red line). (B) Emission spectra of the human recombinant Cb5R with a His6-tag in the absence (black line) and presence of 100 nM of Aβ(1–42) HiLyteTM-Fluor555 (Aβ*555) (red line), and of 100 nM Aβ(1–42) HiLyteTM-Fluor555 (Aβ*555) in the absence of Cb5R (direct excitation spectrum, blue line). Excitation wavelength = 460 nm, excitation and emission slits =10 nm. (C) Emission spectrum of the human recombinant Cb5R with a His6-tag (black line) and the result of the emission spectrum of (Cb5R plus 100 nM of Aβ(1–42) HiLyteTM-Fluor555) after the subtraction of the emission spectrum of 100 nM Aβ(1–42) HiLyteTM- Fluor555 in the absence of Cb5R (red line). Other experimental conditions used for these fluorescence measurements are given in Section 4.