Table 2.

Detection of infectious HEV extracted from naturally infected pig livers. A549/D3 cells were inoculated with suspensions prepared from naturally infected pig livers. A total of 14 livers were tested. Per liver, eight wells of A549/D3 cells were inoculated per dilution (5×, 10× or 100×). The number of positive wells per dilution is indicated between brackets. Log₁₀ viral particles/gram were estimated using the Bayesian MPN model, with a 95% confidence interval (C.I.). Average C_q obtained with RT-qPCR values are given for the viral extract used for cell culture (RIVM) and testing in monitoring studies for the presence of HEV RNA (WFSR). Dilutions and C_q values labelled as n.d. indicate that fluorescence and HEV RNA, respectively, were not detected.

Pig Liver ID	Dilution with Positive IF Results	Log10 Viral Particles/g (95% C.I.)	Cq Values (RIVM)	Cq Values (WFSR)
L-01	5× (8)/10× (3)/100× (1)	2.7 [2.42, 2.96]	31.38	33.75
L-02	n.d.	<0.66	n.d.	35.62
L-03	n.d.	<0.66	31.24	32.17
L-04	n.d.	<0.66	31.09	26.53
L-05	100× (8)	>2.96	25.68	26.53
L-06	100× (8)	>2.96	24.58	24.17
L-07	100× (8)	>2.96	25.52	27.24
L-08	n.d.	<0.66	35.71	35.72
L-09	n.d.	<0.66	32.88	33.25
L-10	n.d.	<0.66	27.17	26.42
L-11	5× (0)/10× (2)/100× (0)	1.5 [0.66, 2.09]	31.18	29.55
L-12	5× (8)/10× (8)/100× (7)	>2.96	27.44	22.15
L-13	n.d.	<0.66	n.d.	n.d.
L-14	n.d.	<0.66	34.66	34.26