TABLE 1.

Composition of the soil master filter

Positiona	Nameb	Mediumc	Sabd	Nearest homologe	cf	kλ/kxg	ςh
1	LQ1	PTYG	0.802	Bordetella bronchiseptica	164	238	20
2	LQ5	PTYG	0.375	Pseudomonas syringae	156	180	10
3	LQ6	PTYG	0.396	Azospirillum sp.	200	283	4
4	LQ10	PTYG	0.598	Sphingomonas parapaucimobilis	110	112	11
5	LQ11	PTYG	0.769	Bacillus macroides	148	46	2
6	LQ14	PTYG	0.615	Xanthomonas campestris	132	61	12
7	LQ15	PTYG	0.580	Bacillus pseudomegaterium	116	68	24
8	LQ16	PTYG	0.861	Pseudomonas syringae	176	95	1
9	LQ17	PTYG	0.931	Agrobacterium rubi	152	112	5
10	LQ19	PTYG	0.349	“Flavobacterium” lutescens	152	157	14
11	LQ20	PTYG	0.816	Pseudomonas syringae	156	210	30
12	LQ21	PTYG	0.791	Bordetella parapertussis	148	294	0
13	LQ26	PTYG	0.865	Bordetella parapertussis	130	166	25
14	LQ27	PTYG		(Bordetella sp.)i	130	149	36
15	LQ29	PTYG	0.843	Bordetella parapertussis	152	208	32
16	LQ30	PTYG	0.935	Sphingomonas yanoikuyae	140	200	5
17	LQ33	PTYG		NDj	180	249	14
18	LQ34	PTYG	0.969	Pseudomonas flavescens	138	170	13
19	LQ35	PTYG	0.972	Pseudomonas flavescens	148	160	6
20	LQ36	PTYG	0.965	Pseudomonas flavescens	136	109	6
21	Q1	HDM, benzene	0.851	Rhodococcus sp.	172	574	98
22	Q2	PTYG	0.879	Bacillus cereus/thuringiensis	148	60	6
23	Q3	PTYG	0.763	Nocardioides luteus	184	330	32
24	Q4	PTYG	0.683	Flavobacterium ferrugineum	160	93	10
25	Q5	HDM, naphthalene	0.779	Pseudomonas syringae	160	142	4
26	Q6	HDM, styrene	0.836	Rhodococcus globerulus	116	202	5
27	Q7	TY	0.741	Pseudomonas syringae	172	151	12
28	Q8	TY	0.879	Bacillus benzoevorans	148	51	7
29	Q9	TY	0.809	Bacillus polymyxa	146	73	7
30	Q10	TY, anoxic	0.504	Bacteroides distasonis	146	72	10
31	Q11	TY, anoxic	0.498	Bacteroides heparinolyticus	240	80	8
32	Q12	TY, anoxic	0.846	Clostridium xylanolyticum	152	65	6
33	Q13	TY, anoxic	0.337	Clostridium sp.	128	61	17
34	Q14	Medium C, anoxic	0.582	Desulfovibrio longus	172	127	6
35	Q15	Medium C, anoxic	0.571	Desulfovibrio desulfuricans	146	94	8

^aPosition of denatured chromosomal DNA on master filter. 

^bName assigned at time of isolation. 

^cMedium used for isolation. Standards 1 to 29 were isolated under aerobic conditions. 

^dSimilarity coefficient for query and matching sequences (10). 

^eNearest homolog in the RDP database as determined by the program SIMILARITY_RANK (10). 

^fAmount of denatured chromosomal DNA (ng) spotted on the filter. 

^gRatio of hybridization constants for bacteriophage λ and genomic DNA (x) from equation 2 (19). 

^hAverage deviation between duplicate measurements of k_λ/k_x. 

ⁱInferred from cross-hybridization with other Bordetella sp. genomes on the filter. 

^jND, not determined.