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. 2023 Oct 12;15(20):4350. doi: 10.3390/nu15204350

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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The expression of acid ceramidase (ASAH1) (A), neutral ceramidase (ASAH2) (B), alkaline ceramidase (ASAH3) (C), sphingosine kinase 1 (SPHK1) (D), and sphingosine kinase 2 (SPHK2) (E) in rat’s liver, as well as the images of representative blots of target protein and corresponding total protein. The expression of enzymes from the ceramide catabolic pathway was assessed by Western blotting, as described in the Section 2. The results are presented as mean ± SD and the data are from n = 6 rats; *—p < 0.05 significant difference vs. control group, #—p < 0.05 significant difference vs. HFHS group.