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Chromosomal deletion of PyrR gene. (A) Schematic diagram of the two steps of pLCNICK-mediated PyrR knockout: the precise cutting PyrR gene in the homologous arm gene editing sites and the deletion pasting of PyrR DNA. (B) Colony PCR assays were conducted using testing primers. (C) The protein expression of PyrR were analyzed via Western blotting, which showed a wild strain of L. casei to be an immunoreactive band and the L. casei ΔPyrR strain to be a non-immunoreactive band.
