TABLE 1.

Results of fermentation experiments done to optimize EPS production by L. delbrueckii subsp. bulgaricus RR

Triala	Runb	Temp (°C)	pH	Bacto-casitone concn (g/liter)	Time to endpoint (h)c	EPS concn (mg/liter)d	ml endpoint
1	6	35	6	30	17.50	268.00	8.20
1	13	35	6	30	17.16	245.68	8.08
2	9	45	5	10	17.80	203.44	7.90
2	14	45	5	10	16.00	169.23	7.98
3	3	35	6	10	26.60	195.93	6.43
3	19	35	6	10	25.50	146.25	6.22
4	12	35	4	30	59.16	268.84	4.35
4	16	35	4	30	58.58	247.54	4.59
5	2	45	6	30	9.42	154.23	8.71
5	10	45	6	30	9.16	181.45	8.96
6	17	40	6	20	12.08	194.23	8.60
7	11	35	5	20	21.16	245.54	7.70
8	8	40	5	30	15.08	279.60	8.27
9	18	35	4	10
10	5	45	4	20	30.40	124.25	6.67
11	15	45	6	20	9.42	139.20	8.86
12	1	40	6	10	14.91	254.31	8.33
13	20	40	4	10	41.00	210.19	5.72
14	7	45	5	30	11.41	178.09	8.29
15	4	35	5	10	38.25	143.34	7.01
16e	21	38	5	30	15.16	354.23	8.34
17f	22	38	5	40	14.00	324.27	8.53

^aEach trial with a different number indicates a unique set of experimental conditions. 

^bRun signifies the order in which the experiments were conducted. 

^cTime at which 90% of the glucose in the growth medium was utilized as detected by high-performance liquid chromatography. 

^dCalculated by subtracting the amount of background interference in uninoculated medium from the amount in fermented broth. 

^eResults of an experiment conducted to verify the predicted optimum conditions. 

^fResults of an experiment conducted with Bacto-casitone at 40 g/liter.