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. 2023 Oct 20;15(20):4459. doi: 10.3390/nu15204459

Table 1.

Characteristics of clinical trials in humans regarding the introduction of mineral-fortified bread into their diet (n = 9).

Jadad
Scale
Participants Fortificant/Substance/
Dose/Inhibitor
Duration Methods/Analysis Results References/
Country
1 Young male adults;
N = 11;
Age: 18–30
BMI: 23.8–28.6
Potassium, Magnesium, Phosphorus;
3.6 g of MgCO3 and 12.5 g of KH2PO4/kg of flour;
7.2 g of MgCO3 and 25 g of KH2PO4/kg of flour
30 days Venous blood;
PG; GR; IR; triacylglycerols; total P, Mg, and K; HOMA-IR
TG and GR after consumption of fortified bread maintained at a lower level compared to control. [14]
Lebanon
3 Mother–child pairs;
N = 22;
Age: 18–45, 2.5–5.
Iron;
2/4 mg of 57Fe as FeFum,
2/4 mg of NaFeEDTA
(Na58FeEDTA and Na57FeEDTA);
2–4 mg/day (4 times during experiment)
28 days Venous blood,
FIA, BIS
FIA from NaFeEDTA is 40% higher than from FeFum; combination of both fortificants does not lead to a significant increase in FIA. [15]
Haiti
1 Women;
N = 46;
Age: 18–49;
Anemic: Hb < 12 g/dL,
Non-anemic:
Hb ≥ 12 g/dL;
BMI: 20.4–25.4
Iron;
6 mg of 57Fe as NaFeEDTA,
6 mg (3 mg/3 mg) of 58Fe/56Fe as NaFeEDTA;
Tea
17 days Venous blood,
FIA
Fe from NaFeEDTA increases by 17% in the anemia-free group and 33% in the iron-deficient group; tea consumption reduced iron absorption (>85%) in both groups. NaFeEDTA cannot overcome the inhibition of tea polyphenols. [16]
Morocco
1 Mother–child pairs;
N = 17;
Age: 18–40, 2–6
BMI: 21.7–22.11.
Iron;
2/4 mg of 58FeSO4,
2/4 mg of 57FeFum;
Tea
30 days Venous blood,
FAFe
Fe absorbed from FeSO4 was 8.85% higher than for FeFum in mothers, and 56.36% in children. Tea reduced FAFe by 44–65%. [17]
Senegal
0 Women;
N = 12;
Age: 33–42
BMI: 25.8–30.1
Iron, Zinc;
58FeSO4,
ZnSO4;
Tea
28 days Venous blood,
CI
Fe absorption from the bread fortified with iron was 19.8% (10.5–37.2%); for 30 mg of Zn/kg, it was 18.5% (10.2–33.4%); 17.7% (7.7–38.7%) for 60 mg of Zn/kg; and 11.2% (6.2–20.3%) for 90 mg of Zn/kg. [18]
Chile
3 Children;
N = 82;
Age: 2–5.
Iron,
Processed amaranth
6 months Venous blood, hemoglobin, serum ferritin, CCR Hemoglobin concentration in the amaranth group increased significantly from 112.1 to 120.3 compared to the control (106.9–113.2 g/L); anemia prevalence: CCR (CI:95%) decrease from 0.57 to 0.37 in amaranth group (μU/mL). [19]
Ethiopia
0 Healthy adults;
N= 12;
Age: 19.6–27.2
BMI: 19.9–24.7
Chromium;
Cr-enriched yeast
500 μg of Cr/50 g of available carbohydrates
4 weeks Venous blood, PG, IR, GR, GI GR after fortified bread was 101.27 mg/dL and 117.82 mg/dL, compared to control (128.46 mg/dL); GI of enriched breads was 34.22 and 62.35 while the control bread had GI = 100. [21]
Greece
3 Type 2 diabetes adults;
N= 30;
Age: 40–65
BMI: 27.9–31.1
Chromium;
Cr-enriched yeast
99.0 μg of Cr/28 g of bread
12 weeks Venous blood,
BMI, GR, IR
Reduction in BMI from 29.3 to 28.9; decreased fasting plasma glucose from 143.2 to 129.6 (mg/dL); decrease of the mean glycosylated hemoglobin from 6.9 to 6.3 (%); decrease in fasting insulin from 6.8 to 4.4 (μU/mL). [22]
Greece
0 Healthy young adults;
Type 2 diabetes adults;
N= 10, 10;
Age: 23–26, 43–65
BMI: 20–26.57
Chromium, Magnesium, Selenium;
Wheat varieties;
Cr: 14.0–15.2 μg/100 g
Mg: 32.9–85.6 mg/100 g
Se: 1.6–5.5 μg/100 g
Buckwheat;
Mg: 172 mg/100 g
Se: 3.5 μg/100 g
2 h Vein blood,
GI,
PG
Lower GI of Jizi439 by 19.67% and 10.78% (that of the mixture of white wheat and Chu20);
the lowest PG in diabetes patients after Jizi439.
[23]
China

Jadad scale—0–5, N—number of population, Age—years, BMI—body mass index (kg/m2), HOMA-IR–homeostasis model assessment of insulin resistance, PG—plasma glucose, P—phosphorus, Mg—magnesium, K—potassium, TG—total glucose, GR—glucose response, IR—insulin response, Fe—iron, FeFum—ferrous fumarate, NaFeEDTA—ethylenediaminetetraacetic acid ferric sodium salt, FIA/FAFe—fractional iron absorption, CI—circulating iron, GI—glycemic index, GR—glycemic response, BIS—body iron stores, Hb—hemoglobin, FIA/FAFe—fractional iron absorption, Zn—zinc, CCR—crude relative risk, CI—confidence interval, Cr—chromium.