Figure 3.

(A) Schematic representation of GEX1, Kar5, and Brambleberry (Bmb). GEX1 has one predicted N-terminal signal sequence (black box), one Cys-rich domain (CRD), two coiled-coil domains (CC), and three transmembrane domains (black boxes). Kar5 and Bmb have two transmembrane domains. (B) Phylogenetic analysis of GEX1 in Viridiplantae. The maximum-likelihood phylogenetic analysis was performed using the amino acid sequences of GEX1 in Viridiplantae. The species and accession numbers of the GEX1 sequences are shown. Saccharomyces cerevisiae Kar5 protein was included as an outgroup. Numbers at nodes indicate bootstrap values calculated from 1000 replicates. The tree is drawn to scale, with branch lengths reflecting the number of substitutions per site. Scale bar, 0.3 substitutions per site.