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. 2023 Oct 18;16(10):1486. doi: 10.3390/ph16101486

Figure 1.

Figure 1

CETSA assay on PAR2 in absence and presence of 1-PPA. (a) PAR2 thermal denaturation performed on live cells shows a decrease in soluble protein as the temperature increases. Data of protein content were initially normalized on Vinculin content and then on the untreated sample (38 °C); (b) 1-PPA at increasing concentration exerts a solubilizing effect on PAR2 even at temperatures higher than the determined Tagg. No effects are exerted on the Vinculin, which remains overall constant for each 1-PPA concentration. Therefore, data were normalized on Vinculin levels and then normalized on the highest concentration of 1-PPA (125 µM).