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. 2023 Oct 17;15(20):4397. doi: 10.3390/nu15204397

Figure 4.

Figure 4

Effect of tributyrin administration on chronic ethanol-induced recruitment of p300-HAT at the CCL2 promoter in the liver: Mice were fed either a control diet (pair-fed, PF) or ethanol (ethanol-fed; EF) (5% v/v)-containing diet. Tributyrin (TB; 2 g/kg) was orally administered to ethanol-fed mice as a preventive (EF + TB (7 + 7)) or interventional (EF + TB (7 + 3)) treatment strategies. ChIP-qPCR quantification from (A) anti-p300 immunoprecipitated chromatin was performed. Differences are expressed as fold-over PF after normalizing for input DNA. (B) Hepatic mRNA analysis for p300 gene expression was performed using real-time PCR after normalizing with the TBP gene and expressed as a fold change over PF. Statistical analysis: mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001 compared with PF or EF via ANOVA with Bonferroni’s test (n = 5–8).