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. 2023 Oct 17;15(20):4397. doi: 10.3390/nu15204397

Figure 6.

Figure 6

Figure 6

Effect of tributyrin administration on transcriptional activation of NFκB in chronic ethanol-fed mice livers: Mice were fed either a control diet (pair-fed, PF) or ethanol (ethanol-fed; EF) (5% v/v)-containing diet. Tributyrin (TB; 2 g/kg) was orally administered to ethanol-fed mice as a preventive (EF + TB (7 + 7)) or interventional (EF + TB (7 + 3)) treatment strategy. (A) Representative immunoblots for nuclear levels of lysine 310 acetylation (Acetyl-p65-K310) and total p65 subunit of NFκB were shown. The densitometric quantification for Acetyl-p65-K310 after normalizing to total p65 and levels of total p65 after normalizing to Lamine B are shown in the bar graphs. (B) Nuclear lysates from mice livers were immunoprecipitated with anti-NFκB-p65 antibodies and immunoblotted with anti-p300, SIRT-1, or NFκB-p65 antibodies. Representative immunoblots are shown. The densitometric quantification was performed, and p300 and SIRT-1 data normalized to NFκB-p65 are shown. (C) Hepatic mRNA analysis for SIRT1 was performed via real-time PCR and shown as fold change over PF. Data are presented as mean ± SEM, and a one-way-ANOVA analysis was used; significance is shown as * p < 0.05, ** p < 0.01, *** p < 0.001 and **** p < 0.0001. Analysis via Student’s t-test is shown as ## p < 0.01.