Figure 10.

Wound healing scratch assay performed on NHDF (a–h) and HSF (i–p) cells, both untreated (CTR) and treated with the advanced medicated electrospun dressing loaded with pirfenidone 1.5% w/w (Biofiber PF), for 24–72 h. The blue dotted lines indicate the wound edges, and the horizontal lines represent the measurement of the wound gap over time. Percentages of wound closure (q,s) and the rate of cell migration (r,t) in a 2D scratch assay are shown, comparing the results obtained from untreated cells (CTR) and cells treated with the advanced medicated dressing containing 1.5% w/w Pirfenidone (Biofiber PF) at different experimental times. The red and light blue lines refer to NHDF and HSF untreated, respectively; statistically significant values are indicated as * p < 0.05, *** p < 0.001. A two-way ANOVA test was performed to evaluate the significance of the data between CTR and cells treated with Biofiber PF.