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. 2023 Oct 11;16(10):1440. doi: 10.3390/ph16101440

Figure 3.

Figure 3

MMP1 was involved in the effects of Cant on inhibiting RA-FLS cell migration and invasion. (A) The contents of MMP1 and MMP3 in the supernatant of Cant-treated (10, 20 μM) RA-FLS cells were detected by ELISA. RA-FLS cells were treated with recombination human MMP1 (1.5 ng/mL) and/or MMP3 (25 ng/mL), the cells were then treated with Cant (20 μM) for 24 h and were subjected to transwell assay (B) and wound-healing assay (C). The migrated or invaded cells in five random fields of each experimental replicate were quantified (D). The statistical chart of the wound-healing results is shown (E). All the experiments were conducted independently three times. The data are shown as mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001, in comparison to RA-FLS treated with TNF-α alone (A). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, in comparison to RA-FLS without TNF-α or Cant treatment (D,E).