Figure 4.

Cant suppressed the expression of YAP/TAZ. (A) RA-FLSs were treated by Cant (10, 20 μM) to detect MST1, LATS1, YAP, and TAZ mRNA levels. (B,C) RA-FLS cells were pre-treated with Cant (10, 20 μM) for 24 h. Then, cells were harvested, and the cell lysates were subjected to Western blot to detect the expression of MST1, LATS1, YAP, TAZ, P-YAP397, and P-YAP127. (D) YAP detected by immunofluorescence staining assay in Cant-treated RA-FLS cells. Magnification, ×20. All the experiments were conducted independently three times. The data are shown as mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, in comparison to RA-FLSs without Cant treatment.