Figure 5.

The treatment with YAP-specific siRNA resulted in reduced inflammatory functions of RA-FLS cells. (A) The effect of si-YAP on the expression of YAP in RA-FLS was measured by Western blot and qPCR. (B,C) Transwell assays and wound-healing tests showed the capacity of the vertical migration, invasion (B), and horizontal migration (C) of si-YAP-treated RA-FLS cells. (D,F) The effect of si-YAP on the levels of MMP1, MMP3, IL-6, and IL-1β in RA-FLS were measured by qPCR (D), and IL-6 in the cell culture supernatant was tested by ELISA (F). (E) Forty-eight hours after si-YAP siRNA transfection, the cells were treated with TNF-α (50 ng/mL). Then, the levels of MMP1 and MMP3 were analyzed by Western blot. All experiments were conducted independently three times. The data are shown as mean ± SEM. * p < 0.05, **** p < 0.0001, in comparison to si-NC RA-FLS group (A–C). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, in comparison to si-NC group with TNF-α treatment (D–F).