Skip to main content
. 2023 Oct 11;16(10):1440. doi: 10.3390/ph16101440

Figure 7.

Figure 7

YAP over-expression significantly reversed Cant-induced decline in RA-FLS cell migration and invasion. (A) The expression of YAP in RA-FLS cells transfected with a lentiviral vector carrying YAP (OE-YAP) or an empty vector (OE-NC) were measured by qPCR and Western blot assay. (B,C) Transwell assays showed the capacity of vertical migration and invasion of Cant-treated OE-NC and OE-YAP RA-FLS cells. (D–G) The effects of Cant on the expression of MMP1, MMP3, IL-6, and IL-1β in OE-NC and OE-YAP RA-FLS cells were measured by qPCR. (H–J) The OE-NC and OE-YAP cells were pre-treated with Cant for 1 h and stimulated with TNF-α (50 ng/mL) for 24 h. Then, the expressions of MMP1 and MMP3 were analyzed by Western blot. (K) IL-6 in the cell culture supernatant was tested by ELISA. (L,M) Western blot detection of proteins in OE-NC and OE-YAP RA-FLS cells after 24 h of Cant stimulation. All experiments were performed independently three times, and data are shown as mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, compared with OE-NC RA-FLS group.