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. 2023 Oct 15;16(10):1465. doi: 10.3390/ph16101465

Figure 3.

Figure 3

The effect of [6]-shogaol on spinal serotonin and relative tryptophan hydroxylase 2 (Tph2) gene expression level in oxaliplatin-injected mice. Changes in spinal serotonin level after oxaliplatin and [6]-shogaol injection (A). Changes in spinal Tph2 gene expression after oxaliplatin and [6]-shogaol injection (B). Changes is serum serotonin level after oxaliplatin and [6]-shogaol injection (C). The level of 5-hydroxytryptamine (5-HT) was measured via enzyme-linked immunosorbent assay (ELISA). Tph2 expression was measured using quantitative real-time polymerase chain reaction (RT-qPCR). Experiments were conducted on the fourth day prior to the injection of oxaliplatin when allodynia was induced in mice. The lumbar 4–5 spinal cords were sampled for the experiment. The control group received 5% glucose. The OXA group received 6 mg/kg of oxaliplatin and 10% of dimethyl sulfoxide (DMSO). The [6]-Shogaol group received oxaliplatin and 10 mg/kg [6]-shogaol. Data are presented as mean ± SD. Control: n = 6; OXA: n = 6; [6]-Shogaol: n = 6. *** p < 0.001, **** p < 0.0001: Control vs. OXA. # p < 0.05; ## p < 0.01: OXA vs. [6]-Shogaol with one-way ANOVA followed by Tukey’s multiple comparisons test.