Table 3.
KD values and binding stoichiometries.
| Peptide/ Peptoid |
LPSEc 1 (µg/mL) |
KD (µM) |
[Binding Sites] (µM) |
Stoichiometry 2 (Peptoid:LPS) |
|---|---|---|---|---|
| Luc-P13#1 | 40 | 0.121 (±0.032) | 4.53 (±0.123) | 1.13:1 |
| Luc-P13#1 | 5 | 0.085 (±0.021) | 2.21 (±0.107) | 4.42:1 |
| Luc-GKY20 | 40 | 0.382 (±0.030) | 6.12 (±0.109) | 1.53:1 |
| Luc-GKY20 | 5 | 0.109 (±0.022) | 2.62 (±0.153) | 5.24:1 |
|
LPSPa 3
(µg/mL) |
||||
| Luc-P13#1 | 5 | 0.032 (±0.0085) | 1.91 (±0.049) | - |
| Luc-GKY20 | 5 | 0.054 (±0.013) | 2.1 (±0.08) | - |
1 LPSEc: LPS from Escherichia coli 0111:B4. 2 Stoichiometry was determined assuming an average MW = 10,000 Da for the E. coli LPS. 3 LPSPa: LPS from Pseudomonas aeruginosa 10.