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. 2023 Oct 20;16(10):1492. doi: 10.3390/ph16101492

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Formation of necrosis and apoptotic cells, and quantitative analysis in the process of burn healing. (A) Immunohistochemical staining for Caspase 3 and HNGB1 in the process of burn healing at day 7. The red box shows the corresponding cell changes of total Caspase 3 and HNGB1 during wound healing. (B,C) Analysis of Caspase 3 and HMGB1 gene expression levels in the process of burn healing at day 7 using qRT-PCR. The images were shown at 20 × 10 magnification (n = 3; scale bars = 50 µm; * p < 0.05, ** p < 0.01, compared with the PBS treatment; ^# p < 0.05, compared with the hFGF2 treatment).