TABLE 1.
Secretion of enzymes as a function of growth pH in minimal medium alone or supplemented with cuticle
| pH | Enzyme (optimum pH) activitya
|
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| NAGase (5)
|
Pr1 (8)
|
Pr2 (8)
|
Aminopeptidase (7)
|
Aspartyl protease (3)
|
||||||
| −C | +C | −C | +C | −C | +C | −C | +C | −C | +C | |
| 3 | 17 ± 3 (59) | 18 ± 4 (42) | 0 (0) | 0 (0) | 0 (0) | 0.1 (0) | 0 (0) | 0 (0) | 19 ± 3 (90) | 72 ± 5 (85) |
| 4 | 23 ± 4 (78) | 36 ± 5 (83) | 4 ± 1 (6) | 30 ± 3 (13) | 4 ± 1 (13) | 4 ± 1 (7) | 0 (0) | 0 (0) | 21 ± 2 (100) | 85 ± 4 (100) |
| 5 | 29 ± 4 (100) | 43 ± 6 (100) | 15 ± 2 (22) | 63 ± 5 (28) | 8 ± 1 (27) | 23 ± 3 (38) | 0 (0) | 0 (0) | 7 ± 1 (33) | 27 ± 2 (32) |
| 6 | 27 ± 3 (92) | 40 ± 4 (94) | 36 ± 4 (52) | 165 ± 8 (72) | 20 ± 2 (67) | 53 ± 3 (87) | 2.8 ± .5 (30) | 5.5 ± 1 (40) | 2 ± 0 (10) | 13 ± 1 (15) |
| 7 | 14 ± 3 (49) | 15 ± 2 (35) | 39 ± 6 (57) | 175 ± 8 (77) | 30 ± 4 (100) | 55 ± 4 (90) | 9.2 ± 1 (100) | 14 ± 1 (100) | 1 ± 0 (5) | 7 ± 1 (8) |
| 8 | 12 ± 2 (41) | 13 ± 2 (29) | 69 ± 7 (100) | 228 ± 11 (100) | 25 ± 3 (83) | 61 ± 5 (100) | 3.4 ± 1 (37) | 10 ± 2 (73) | 0 (0) | 3 ± 0.2 (4) |
a
Activity is expressed as nanomoles of NP per minute per milliliter (for NAGase), nanomoles of NA per minute per milliliter (for Pr1, Pr2, and aminopeptidase), or change in optical density per 10 min per milliliter (for aspartyl protease), followed by the percent of maximum activity of the enzyme (in parentheses). −C, minimal medium alone; +C, minimal medium supplemented with 1% cockroach cuticle.