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Biophysical characterization of five recombinant RBD proteins.
| RBD Variant | Purification Yield [RBD (mg)/Fermentation Supernatant (L)] |
SDS-PAGE with Coomassie Blue Staining (Non-Reduced) | Homogeneity via SE-HPLC (%) | Radius via Dynamic Light Scattering (nm) |
|
|---|---|---|---|---|---|
| MW (kDa) | Purity (%) | ||||
| WT-RBD | 270.5 | 25.3 | 94.1 | 99.7 | 2.57 ± 0.01 |
| Beta-RBD | 204.3 | 23.8 | 93.2 | 99.7 | 2.63 ± 0.01 |
| Delta-RBD | 294.5 | 25.1 | 93.9 | 98.4 | 2.73 ± 0.02 |
| BA.4/5-RBD | 46.2 | 24.7 | 94.2 | 96.4 | 2.58 ± 0.02 |
| XBB.1.5-RBD | 78.0 | 25.1 | 93.2 | 99.4 | 2.71 ± 0.03 |
