Table 1.
Forward and reverse sequences for genes related to cell death, the inflammasome complex and antioxidant biomarkers.
| Gene | Forward | Reverse |
|---|---|---|
| Cell Death | ||
| BAX (BCL-2-associated X protein) | CCCCCGAGAGGTCTTTTTCC′ | CCTTGAGCACCAGTTTGCTG′ |
| CASP3 (caspase 3) | ‘GTGGAGGCCGACTTCTTGTA′ | TTTCAGCATGGCACAAAGCG′ |
| APAF1 (apoptotic protease-activating factor 1) | TCTTCCAGTGGTAAAGATTCAGTT′ | CGGAGACGGTCTTTAGCCTC′ |
| BNIP3 (BCL2-interacting protein 3) | CCTCAGCATGAGGAACACGA′ | GCCACCCCAGGATCTAACAG′ |
| BCL2 (B-cell lymphoma 2) | ′TCTCATGCCAAGGGGGAAAC′ | TCCCGGTTATCGTACCCTGT′ |
| P53 (tumor protein P53) | GAACAAGTTGGCCTGCACTG | GAAGTGGGCCCCTACCTAGA |
| AKT1 (AKT serine/threonine kinase 1) | GAAGGACGGGAGCAGGC | TGTACTCCCCTCGTTTGTGC |
| Inflammasome Complex | ||
| NLRP3 (NLR pyrin domain containing 3) | CCCCGTAATCAACGGGACAA′ | AGCCAAATGCTTACCAGAAAGT′ |
| CASP1 (caspase 1) | ′GAAAAGCCATGGCCGACAAG′ | GCCCCTTTCGGAATAACGGA′ |
| IL1β (interleukin-1 beta) | ′CCAGCTACGAATCTCCGACC′ | TATCCTGTCCCTGGAGGTGG |
| NFκB (nuclear factor kappa B) | ′TTTTCGACTACGCGGTGACA′ | GTTACCCAAGCGGTCCAGAA′ |
| TNFα (tumor necrosis factor alpha) | CTGGAAAGGACACCATGAGCA′ | TCTCTCAGCTCCACGCCATT′ |
| IL6 (interleukin 6) | ‘CCAGTACCCCCAGGAGAAGA′ | CAGCTCTGGCTTGTTCCTCA′ |
| Antioxidant Biomarkers | ||
| NRF2 (nuclear factor erythroid related factor2) | CTGGTCATCGGAAAACCCCA′ | TCTGCAATTCTGAGCAGCCA’ |
| SOD (superoxide dismutase) | ′CCACTGCTGGGGATTGATGT′ | CGTGGTTTACTTTTTGCAAGCC′ |
| GPx (glutathione peroxidase) | ′TTCGAGCCCAACTTCATGCT′ | ′CGATGTCAGGCTCGATGTCA′ |
| Normalizer | ||
| GAPDH (glyceraldehyde-3-phosphate dehydrogenase) | ′GAGAAGGCTGGGGCTCATTT | AGTGATGGCATGGACTGTGG′ |