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. 2023 Sep 29;11(10):1546. doi: 10.3390/vaccines11101546

Figure 1.

Figure 1

Construction and production of OMVs carrying SARS-CoV-2 RBM antigens. (A) Topology of the interaction between SARS-CoV-2 RBD and ACE2 with the indication of the two RBM polypeptides tested in this study. (B) Schematic representation of the pET21-based constructs expressed in BL21(DE3)Δ60 to decorate OMVs. Staphylococcus aureus Ferric hydroxamate receptor 2 (FhuD2) was fused to one copy of RBM438–462, RBM467–509 and RBM438–509 SARS-CoV-2 epitopes. (C) SDS-PAGE of purified OMVs derived from BL21(DE3)Δ60 cultures expressing pET21-FhuD2-RBM438–462, pET21-FhuD2-RBM467–509 and pET21-FhuD2-RBM438–509 plasmids or the control pET21-FhuD2 or empty pET21 plasmids. Stars indicate the predicted migration of the fusion proteins.