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. 2023 Oct 27;9(43):eadh3273. doi: 10.1126/sciadv.adh3273

Fig. 3. Mechanically stimulated ORS cells evoke calcium transients in LTMRs.

Fig. 3.

(A) Images showing LTMR calcium transients before and after ORS cell activation (baseline and stim, respectively). (B) Representative traces of ΔF/F in LTMRs after mechanical activation of ORS cells. (C) Correlation of time to calcium transient in LTMR with distance from activated ORS cell. (D) Images of LTMRs with or without bafilomycin after ORS cell activation. (E) Mean ΔF/F in LTMRs with or without bafilomycin after ORS stimulation. (F) Graph showing peak ΔF/F in n = 2 LTMR cultures from one patient (26 different cells under each condition). An unpaired t test was used to compare the ΔF/F in all cells between the control and drug-treated conditions. (G) Images of LTMRs with or without apyrase after ORS cell stimulation. (H) Mean ΔF/F in LTMRs with or without apyrase after ORS activation. (I) Graph showing peak ΔF/F in n = 4 LTMR cultures from three patients (total 33 and 27 cells under each condition, respectively). An unpaired t test was used to compare the peak ΔF/F in all cells from each condition. The dashed arrowheads in (A), (D), and (G) show the point of stimulus. Scale bars, (A, D, and G) 50 μm. ***P < 0.001