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. 2023 Oct 10;11(10):847. doi: 10.3390/toxics11100847

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Evaluation of global DNA methylation and histone modulations in BEAS-2B cells exposed to HTP, e-cig and cigarette emissions. Cells were exposed to 120 puffs of HTP and e-cig (Mb-18W and Mb-30W) or to 4 puffs of 3R4F cigarette. (A) Global DNA methylation was measured 24 h after exposure. Results are expressed as percentages of global methylation. (B) Modifications of histones (H3K9me3, H3K9ac, H3K4me3 and H3K27me3) were measured by ELISA 24 h after exposures. Results are expressed as the ratio of modified histone/total H3. Data represent the median from three independent culture replicates. NS: not significant.