Table 2.

The physical properties and hemolytic activity of Protopolybia-MPIII and mutants.

Peptides	Sequences	pI	<H>	<μH>	Z	HRBC		RRBC
						EC50 (μM)	R2	EC50 (μM)	R2
Protopolybia-MPIII	INWLKLGKAVIDAL-NH2	8.59	0.674	0.506	2	23.0 ± 1.2	0.9968	42.8 ± 2.1	0.991
Protopolybia-MPI	INWLKLGKKVSAIL-NH2	10.30	0.634	0.439	4	-	-	-	-
Protopolybia-MPIII-1	INWLKLGKKVIDAL-NH2	9.70	0.581	0.580	3	176.4 ± 1.0	0.9999	158.4 ± 2.3	0.9996
Protopolybia-MPIII-2	INWLKLGKAVSDAL-NH2	8.59	0.543	0.453	2	673.3 ± 189.0	0.9999	639.5 ± 1.6	0.9998
Protopolybia-MPIII-3	INWLKLGKAVIAAL-NH2	10.00	0.751	0.434	3	16.8 ± 1.0	0.9959	21.5 ± 1.2	0.9963
Protopolybia-MPIII-4	INWLKLGKAVIDIL-NH2	8.59	0.781	0.497	2	11.3 ± 6.2	0.9021	19.8 ± 1.2	0.9495
Protopolybia-MPIII-5	INWLKLGKKVSDAL-NH2	9.70	0.450	0.513	3	-	-	-	-
Protopolybia-MPIII-6	INWLKLGKKVIAAL-NH2	10.30	0.659	0.512	4	110.0 ± 2.5	0.9992	107.6 ± 3.2	0.9927
Protopolybia-MPIII-7	INWLKLGKKVIDIL-NH2	9.70	0.688	0.560	3	37.8 ± 3.7	0.9908	57.5 ± 2.7	0.9967
Protopolybia-MPIII-8	INWLKLGKAVSAAL-NH2	10.00	0.620	0.377	3	307.8 ± 6.2	0.9999	444.0 ± 2.1	0.9999
Protopolybia-MPIII-9	INWLKLGKAVSDIL-NH2	8.59	0.649	0.473	2	167.3 ± 2.7	0.9994	161.7 ± 1.3	0.9996
Protopolybia-MPIII-10	INWLKLGKAVIAIL-NH2	10.00	0.858	0.421	3	11.3 ± 1.2	0.9899	11.7 ± 1.0	0.9883
Protopolybia-MPIII-11	INWLKLGKAVSAIL-NH2	10.00	0.726	0.396	3	140.6 ± 2.4	0.9996	133.6 ± 2.2	0.9993
Protopolybia-MPIII-12	INWLKLGKKVIAIL-NH2	10.30	0.765	0.486	4	43.9 ± 3.6	0.9928	55.2 ± 2.2	0.9978
Protopolybia-MPIII-13	INWLKLGKKVSDIL-NH2	9.70	0.556	0.516	3	-	-	-	-
Protopolybia-MPIII-14	INWLKLGKKVSAAL-NH2	10.30	0.527	0.439	4	-	-	-	-

-: the hemolytic activity was too low to calculate the EC₅₀.