Figure 3.

Effect of CTX on the number of cells obtained from EAT in mice. The animals were inoculated with EAT (1 × 10⁷ cells/0.5 mL PBS) or injected, i.p., with PBS and treated concomitantly with different doses of CTX (0.9 µg/animal and 5.0 µg/animal in 100 µL of saline, s.c.) or saline (100 µL). On the 6th or 13th day of the EAT inoculation, the total counts (A,D) of tumor cells (B,E) and leukocytes (C,F) were determined from the ascitic fluid and the peritoneal cavity (non-carrier animals) in a Neubauer hemocytometer. Values are expressed as mean ± SEM of 3–9 animals per group. * p < 0.05 compared to the CTX-treated group. ** p < 0.01 compared to the control group (EAT + saline). *** p < 0.001 compared to the control group (EAT + saline) and the CTX-treated group. # p < 0.05 compared to the control group (PBS + saline).