Table 1.

Effect of CTX on cytokine secretion from the peritoneal cavity of non-tumor-bearing animals and on the ascitic fluid of tumor-bearing animals after 6th day of inoculation of EAT cells.

6th Day after EAT Inoculation
	Non-Tumor-Bearing Mice			Tumor-Bearing Mice
Cytokines (pg/mL)	Saline	CTX 0.9 µg/Animal	CTX 5.0 µg/Animal	Saline	CTX 0.9 µg/Animal	CTX 5.0 µg/Animal
IL-1β	14.5 ± 1.50	15.8 ± 3.04	14.0 ± 3.24	7.3 ± 1.65	8.0 ± 2.04	6.5 ± 0.87
IL-10	13.1 ± 10.7	9.2 ± 9.2	30.6 ± 15.1	84 ± 29.8	N.D. *	N.D. *
TNF-α	252.8 ± 37	247.5 ± 57.2	297.3 ± 22	114.5 ± 30.4	65.7 ± 4.8	50.1 ± 8.2

Animals were injected, i.p., with PBS (non-tumor-bearing animals) or inoculated (tumor-bearing animals) with EAT (1 × 10⁷ cells/0.5 mL PBS) and treated concomitantly with different doses of CTX (0.9 µg/animal and 5.0 µg/animal in 100 µL saline, s.c.) or saline (100 µL-control). After the 6th day of PBS or EAT inoculation, peritoneal lavage or ascitic fluid, respectively, from each group was collected to determine the dose of cytokines (IL-1β; IL-10 and TNF-α) by ELISA. Values are expressed as mean ± SEM of 4 animals per group. * p < 0.05, compared to the control group (PBS + saline; EAT + saline).