Table 2.

Effect of CTX on the cytokine secretion from the peritoneal cavity of non-tumor-bearing animals and on the ascitic fluid of tumor-bearing animals after the 13th day of inoculation of EAT cells.

13th Day after EAT Inoculation
	Non-Tumor-Bearing Mice			Tumor-Bearing Mice
Cytokines (pg/mL)	Saline	CTX 0.9 µg/Animal	CTX 5.0 µg/Animal	Saline	CTX 0.9 µg/Animal	CTX 5.0 µg/Animal
IL-1β	13.8 ± 3.8	13.3 ± 3.7	13.7 ± 0.24	5.8 ± 1.44	3.5 ± 1.85	2.5 ± 0.50
IL-10	69.8 ± 68.8	42.6 ± 42.6	1.9 ± 1.9	342 ± 75	309 ± 61	267 ± 54
TNF-α	298 ± 99	167 ± 44	215 ± 7.3	14.3 ± 3	18 ± 5	24 ± 1.3

Animals were injected, i.p., with PBS (non-tumor-bearing animals) or inoculated (tumor-bearing animals) with EAT (1 × 10⁷ cells/0.5 mL PBS) and treated concomitantly with different doses of CTX (0.9 µg/animal and 5.0 µg/animal in 100 µL saline, s.c.) or saline (100 µL-control). After the 13th day of PBS or EAT inoculation, peritoneal lavage or ascitic fluid, respectively, from each group was collected to determine the dose of cytokines (IL-1β; IL-10 and TNF-α) by ELISA. Values are expressed as mean ± SEM of 4 animals per group.