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. 2023 Oct 17;11(10):1604. doi: 10.3390/vaccines11101604

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Vδ2 functional responses after ID BCG + aerosol BCG boost. (a) Intracellular cytokine staining (ICS) was utilised to determine the proportion of Vδ2 cells producing IFN-γ, TNF-α or IL-17 in the circulation (dark-green box plots) or in the BAL (light-green box plots) prior to and after an aerosol BCG boost. Results are from ex vivo BCG-stimulated wells minus results from wells without ex vivo stimulation. (b) Log2 fold changes in the proportion of Vδ2 cells expressing CD107a, or producing granulysin, or granzyme B, from baseline. Shown are results without any ex vivo stimulation, and with an ex vivo BCG stimulation, minus results in unstimulated cells. Asterisks represent significant differences between indicated timepoints, with * = p < 0.05 and ** = p < 0.01.