Table 1.
Human NatC substrates identified in HAP1 cells using SCX-based N-terminal proteomics
| Protein | UniProt ID | P1' | P2' | Start | Modificationa | Detected peptide | Median Nt-acetylation (%)b | Protein abundance WT vs NatC KOc | |||
|---|---|---|---|---|---|---|---|---|---|---|---|
| WT | 30-KO | 35-KO | 38-KO | ||||||||
| SSBP3 | Q9BWW4 | M | F | 1 | Ac | MFAKGKG.. | 99.4 | - | - | - | nd |
| AcDC | - | - | 9.1 | - | |||||||
| EBF3 | Q9H4W6 | M | F | 1 | Ac | MFGIQEN.. | 100.0 | - | - | - | nd |
| NUP133 | Q8WUM0 | M | F | 1 | Ac | MFPAAP.. | 99.9 | - | - | - | Depleted |
| ADNP2 | Q6IQ32 | M | F | 1 | Ac | MFQIPVE | 98.9 | - | - | - | nd |
| ADNP | Q9H2P0 | M | F | 1 | Ac | MFQLPVN.. | 99.8 | - | - | - | nd |
| RPS7 | P62081 | M | F | 1 | Ac | MFSSSAK.. | 76.9 | - | - | - | nd |
| AcDC | 77.3 | 25.0 | - | 22.3 | |||||||
| UBE2M | P61081 | M | I | 1 | Ac | MIKLFSL.. | 99.0 | - | - | - | Depleted |
| DPYSL5 | Q9BPU6 | M | L | 1 | Ac | MLANSA.. | 98.8 | - | - | - | Depleted |
| IST1 | P53990 | M | L | 1 | Ac | MLGSGFK.. | 92.1 | - | - | - | nd |
| AcDC | - | 3.1 | 8.1 | 5.3 | |||||||
| ATXN2L | Q8WWM7 | M | L | 1 | Ac | MLKPQPL.. | 99.5 | - | - | - | Enriched |
| DIS3 | Q9Y2L1 | M | L | 1 | Ac | MLKSKTF.. | 99.1 | - | - | - | Depleted |
| MAP7D3 | Q8IWC1 | M | M | 1 | Ac | MMADGAA.. | 99.7 | - | - | - | nd |
| ANK2 | Q01484 | M | M | 1 | Ac | MMNEDAA.. | 100.0 | - | - | - | Depleted |
| ITM2A | O43736 |
M M |
V V |
1 2 |
Ac AcDC |
MVKIAFN.. VKIAFNT.. |
99.0 0.1 |
- 0.7 |
- - |
- 0.1 |
nd |
| SSBP4 | Q9BWG4 | M | Y | 1 | Ac | MYAKGGK.. | 99.4 | nd | |||
| MCRIP2 | Q9BUT9 | M | Y | 1 |
Ac AcDC |
MYTITKG.. |
99.9 - |
- 34.5 |
- - |
37.5 34.5 |
nd |
nd not detected.
aIn vivo (Ac) and in vitro (AcDC) acetylated N-termini.
bMedian Nt-acetylation percentage in HAP1 WT, NAA30-KO, NAA35-KO, NAA38-KO cells is indicated (n = 4, Supplementary Data 3).
cChange in protein abundance between WT and at least one NatC KO cell line determined by LFQ proteomics (Supplementary Data 4).