Table 1.
ddPCR and qRT-PCR assay sequences
| RAG2 KO (DNA) | Forward | CTCTCCCAGAGCAACAAAGAC |
| Reverse | TTTCCATGCCTTGCAAAATGG | |
| Probe | 56-FAM/CCTACAGGT/ZEN/GGGGTCTTTCATTCC/3IABkFQ | |
| RAG2 KI Correction (DNA) | Forward | TCTCCCAGAGCAACAAAG |
| Reverse | GAAGTTCATGAGGCTGAAG | |
| Probe | 56-FAM/CATAGCCTT/ZEN/AATCCAGCCCG/3IABkFQ | |
| RAG1 KO (DNA) | Forward | CAGTGACTTTCAGGATGACCT |
| Reverse | CAGCTAGCTTGCCAAACC | |
| Probe | 56-FAM/CCATGCTGG/ZEN/CTGAGGTACC/3IABkFQ | |
| CCRL2 (DNA) | Forward | GCTGTATGAATCCAGGTCC |
| Reverse | CCTCCTGGCTGAGAAAAAG | |
| Probe | 5HEX/TGTTTCCTC/ZEN/CAGGATAAGGCAGCTGT/3IABkFQ | |
| Endogenous RAG2 (RNA) | Forward | CCAAGTGCTGACAATTAATACCTG |
| Reverse | GACATGGTTATGCTTTACATCCAG | |
| Probe | 56-FAM/CATCAGTGA/ZEN/GAAGCCTGGCTGAATTAAGG/3IABkFQ | |
| dcoRAG2 cDNA (RNA) | Forward | CAAGTGCTGACAATTAATACCT |
| Reverse | GAAGTTCATGAGGCTGAAG | |
| Probe | 56-FAM/CATAGCCTT/ZEN/AATCCAGCCCG/3IABkFQ | |
| Comparison RAG2 (RNA) | Forward | CCAAGTGCTGACAATTAATACCTG |
| Reverse | GACATAGTTTCTGATGGTACGTAGA | |
| Probe | 56-FAM/TCACGCCTC/ZEN/TCTGAATCTTTGCCG/3IABkFQ | |
| GAPDH (RNA) | Forward | ACATCGCTCAGACACCATG |
| Reverse | TGTAGTTGAGGTCAATGAAGGG | |
| Probe | 56-FAM/AAGGTCGGA/ZEN/GTCAACGGATTTGGTC/3IABkFQ |