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Replication of ASFV-G-ΔI177L in successive passages in IPKM cells. ASFV-G-ΔI177L and ASFV-G were passed 10 consecutive times (MOI = 1) in IPKM cell cultures. Viral yield in each passage was quantified in primary cultures of swine macrophages and titers expressed as HAD50/mL. Titrations were conducted by duplicated (presented data represent one set of them).
