TABLE 1.
Purification step | Total protein (mg) | Total activity (U) | Sp act (U/mg) | Purifi- cation factor | Yield (%) |
---|---|---|---|---|---|
Culture supernatant | 103.4 | 68.7 | 0.66 | 1 | 100 |
(NH4)2SO4 fractionation | 56 | 51.4 | 0.91 | 1.37 | 74.8 |
Q Sepharose fast flow | 22.5 | 47.2 | 2.1 | 3.18 | 68.7 |
Phenyl Sepharose HR | 1.65 | 20.2 | 12.2 | 18.5 | 29.4 |
Q Sepharose fast flow | 0.35 | 12.5 | 35.9 | 54.4 | 18.2 |
Sephacryl HR200 | 0.065 | 7.72 | 118.8 | 179.2 | 11.2 |