TABLE 1.

Purification of B. pumilus AXE

Purification step	Total protein (mg)	Total activity (U)	Sp act (U/mg)	Purifi- cation factor	Yield (%)
Culture supernatant	103.4	68.7	0.66	1	100
(NH4)2SO4 fractionation	56	51.4	0.91	1.37	74.8
Q Sepharose fast flow	22.5	47.2	2.1	3.18	68.7
Phenyl Sepharose HR	1.65	20.2	12.2	18.5	29.4
Q Sepharose fast flow	0.35	12.5	35.9	54.4	18.2
Sephacryl HR200	0.065	7.72	118.8	179.2	11.2