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. 2023 Oct 28;22:222. doi: 10.1186/s12934-023-02215-x

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 7 — Metabolic engineering of S. rimosus for heterologous production of the polyketide bhimamycin. LC‒MS analysis of crude extract from S. rimosus HP126 Δv3 and S. rimosus HP126, showing the extracted ion chromatogram for 461.15–461.20 Da (RT 2.62), which reveals the absence of oxytetracycline in the deletion strain (A). Chemical structure of bhimamycin (B). Final levels of the product from cultures of S. rimosus ATCC 10970, S. rimosus HP126, and S. rimosus Δv3 (C).