TABLE 2.
Theoretical alignment of sequences of amplimers 63f and 1387r with database sequences of 16S rRNA genes from species not tested by PCR
| Strain | Woese grouping | Sequence vsa:
|
|
|---|---|---|---|
| 63f | 1387r | ||
| CAGGCCTAACACATGCA—AGTC | CGGAACATGTGWGGCGGG | ||
| Fibrobacter intestinales | Fibrobacter spp. | AGTGTCTTATACATGCA—AGTC | GCCTTGTACACACCGCCC |
| Chlorobium limicola | Green non-sulfur | CGTGCCTAACACATGCA—AGTC | GCCTTGTACACACCGCCC |
| Chloroflexus aurantiacus | Green non-sulfur | CGTGCCTAATGCATGCA—AGTC | GCCTTGTACACACCGCCC |
| Herpetosiphon aurantiacus | Green non-sulfur | CGTGCCTAATGCATGCA—AGTC | GCCTTGTACACACCGCCC |
| Thermomicrobium roseum | Green non-sulfur | CGTGCCTAATGCATGCA—AGTC | GCCTTGTACACACCGCCC |
| Deinococcus radiodurans | Radioresistant micrococci and relatives | CGTGCTTAAGACATGCA—AGTC | GCCTTGTACACACCGCCC |
| Thermus aquaticus | Radioresistant micrococci and relatives | CGTGCCTAAGACATGCA—AGTC | GCCTTGTACACACCGCCC |
| Thermus ruber | Radioresistant micrococci and relatives | TATGCCTAAGACATGCA—AGTC | GCCTTGTACACACCGCCC |
| Fervidobacterium nodosum | Thermotogales | CGTGCCTAACACATGCA—AGTC | GCCTTGTACACACCGCCC |
| Thermotoga elfii | Thermotogales | CGTGCTTAACACATGCA—AGTC | GGTTTGCACACACCGCCC |
| Thermotoga maritima | Thermotogales | CGTGCCTAACACATGCA—AGTC | GCCTTGTACACACCGCCC |
a
Nucleotides underlined and in boldface differ from corresponding nucleotides in amplimer.