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. 2023 Oct 28;23:524. doi: 10.1186/s12870-023-04522-2

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 9 — Reduced drought tolerance of TRV2:CePP2C19 in tiger nut. A Drought stress with 30% PEG6000 on TRV2:CePP2C19 and TRV2:00 plants. B qRT-PCR analysis of CePP2C19 expression in leaves of TRV2:00 and TRV2:CePP2C19 plants exposed to drought stress. Relative expression level of each gene was normalized to that of the tiger nut ADF7 (CeADF7) as a standard control. Error bars represent the standard deviation. * Significant difference at P < 0.05 *** significant difference at P < 0.001. C qRT-PCR analysis of CeSnRK2.3 expression in leaves of TRV2:00 and TRV2:CePP2C19 plants exposed to drought stress. Relative expression level was normalized to that of the tiger nut ADF7 (CeADF7) as a standard control. Error bars represent the standard deviation. **significant difference at P < 0.01 *** significant difference at P < 0.001 (D) Determination of ABA content in TRV2:00 and TRV2:CePP2C19. Error bars represent the standard deviation. *Significant difference at P < 0.05